Entrainment of Cerebellar Cells with tACS in Rats

Share on facebook
Share on twitter
Share on google
Share on linkedin
Share on email
Share on print
nathan whitmore and his rats
Link -

Entrainment of Cerebellar Purkinje Cells with Directional AC Electric Fields in Anesthetized Rats.

BACKGROUND: Transcranial electrical stimulation (tES) shows promise to treat neurological disorders. Knowledge of how the orthogonal components of the electric field (E-field) alter neuronal activity is required for strategic placement of transcranial electrodes. Yet, essentially no information exists on this relationship for mammalian cerebellum in vivo, despite the cerebellum being a target for clinical tES studies.
OBJECTIVE: /Hypothesis: To characterize how cerebellar Purkinje cell (PC) activity varies with the intensity, frequency, and direction of applied AC and DC E-fields.
METHODS: Extracellular recordings were obtained from vermis lobule 7 PCs in anesthetized rats. AC (2-100 Hz) or DC E-fields were generated in a range of intensities (0.75-30 mV/mm) in three orthogonal directions. Field-evoked PC simple spike activity was characterized in terms of firing rate modulation and phase-locking as a function of these parameters. t-tests were used for statistical comparisons.
RESULTS: The effect of applied E-fields was direction and intensity dependent, with rostrocaudally directed fields causing stronger modulations than dorsoventral fields and mediolaterally directed ones causing little to no effect, on average. The directionality dependent modulation suggests that PC is the primary cell type affected the most by electric stimulation, and this effect was probably given rise by a large dendritic tree and a soma. AC stimulation entrained activity in a frequency dependent manner, with stronger phase-locking to the stimulus cycle at higher frequencies. DC fields produced a modulation consisting of strong transients at current onset and offset with an intervening plateau.
CONCLUSION: (s): Orientation of the exogenous E-field critically determines the modulation depth of cerebellar cortical output. With properly oriented fields, PC simple spike activity can strongly be entrained by AC fields, overriding the spontaneous firing pattern.

PMID: 32919090 [PubMed – as supplied by publisher]

Brain Stimul. 2020 Sep 09;:

Authors: Asan AS, Lang EJ, Sahin M

Join Our Newsletter



Hi, I'm the foc.us Research Bot. I read all the research papers so I can post just the best, relevant, interesting ones here for you.


Leave a Reply

About Author

Hi, I’m the foc.us Research Bot. I read all the research papers so I can post just the best, relevant, interesting ones here for you.

Recent Posts

Follow Us

Weekly Tutorial